RT-PCR | | |
RBP2 | 5′-CCTCCATTTGCCTGTGAAGT-3′ | 5′-CCTTTGCTGGCAACAATCTT-3′ |
PLU-1 | 5′-CTTCTTGTTTGCCTGCATCA-3′ | 5′-ATTTTGGGATTTCCCTCCAC-3′ |
SMCX | 5′-GGCCAAAGACAAGACTCTGC-3′ | 5′-CCGTAGCCTCATGGTCATCT-3′ |
SMCY | 5′-GCTAAGGGCACTGGAGTCTG-3′ | 5′-TCAAGGTCAGCTGTGGAGTG-3′ |
LSD1 | 5′-CAAGTGTCAATTTGTTCGGG-3′ | 5′-TTCTTTGGGCTGAGGTACTG-3′ |
Sp1 | 5′-GGCGAGAGGCCATTTATGTGT-3′ | 5′-TGCATGACGTTGATGCCACT-3′ |
KLF4 | 5′-ACGATCGTGGCCCCGGAAAAGGACC-3′ | 5′-TGATTGTAGTGCTTTCTGGCTGGGCTCC-3′ |
E-cadherin, CDH1 | 5′-TTTCTTGGTCTACGCCTGGGACTC-3′ | 5′-CACCTTCAGCCATCCTGTTTCTC-3′ |
β-Actin | 5′-ACACTGTGCCCATCTACGAGG-3′ | 5′-AGGGGCCGGACTCGTCATACT-3′ |
Cloning of plasmids | | |
RBP2 promoter | 5′-GGGGTACCGGGGATGGGTTAGACCTGCACTT-3′ | 5′-GAAGATCTTTCTTCTCTTCCCCGGCAGCAC-3′; |
PLU-1 promoter | 5′-GGGGTACCTCAATAAAAGTTGGCTCAAC-3′ | 5′-GAAGATCTAACAGCAAGTCCGAGTTGTA-3′ |
LSD1 promoter | 5′-GGGGTACCGTCACCTTCGGAGGTTTAGTC-3′ | 5′-GAAGATCTCTTCTTCCCAGATAACATCTCGG-3′ |
Chromatin immunoprecipitation | | |
KLF4 promoter | 5′-GCCTTCTCTTGAGGCTCCCAGTTCA-3′ | 5′-TTATCCGCGTGACTCATCCAGCCC-3′ |
E-cadherin CDH1-promoter | 5′-GAGAGTCTCTTGAACCCGGCAG-3′ | 5′-TGTAGAGAGACAAGTCGGGGCG-3′ |
RBP2 promoter | 5′-GGGGATGGGTTAGACCTGCACTT-3′ | 5′-TTCTTCTCTTCCCCGGCAGCAC-3′ |
PLU-1 promoter | 5′-TCAATAAAAGTTGGCTCAAC-3′ | 5′AACAGCAAGTCCGAGTTGTA-3′ |
LSD-1 promoter | 5′-GTCACCTTCGGAGGTTTAGTC-3′ | 5′-CTTCTTCCCAGATAACATCTCGG-3′. |