Calculation of bias factors for norepinephrine and epinephrine at the α1A-AR
LogTR is the log of the transduction ratio (τ/KA), and logTRn values are normalized to the logTR for norepinephrine. To avoid propagation of error due to multiple data manipulation steps, Student's t tests were carried out on the raw logTR data rather than data normalized to norepinephrine (see Supplemental Table 1). P < 0.05 was considered significant.
| logTRn [pathway 1-pathway 2] | |||
|---|---|---|---|
| [cAMP–Ca2+] (Bias factor) | [ECAR–cAMP] (Bias factor) | [ECAR–Ca2+] (Bias factor) | |
| Norepinephrine | 0.00 ± 0.20 (1.0) | 0.00 ± 0.18 (1.0) | 0.00 ± 0.21 (1.0) |
| Epinephrine | −0.36 ± 0.20 (0.43) | 0.26 ± 0.17 (1.8) | −0.10 ± 0.20 (0.79) |