Competition between 1 μM SNC80 or nociceptin/OFQ and other Gi/o-coupled receptor agonists for short-term inhibition of AC
Inhibition of AC by 1 μM of agonist(s) alone or in combination was performed as described for Fig. 1 and under Materials and Methods. Percentage competition was calculated from three individual experiments, in duplicate, as described for Table 2.
| Agonist R1 (Receptor) | Agonist R2 (Receptor) | Competition |
|---|---|---|
| % | ||
| SNC80 (DOR) | Nociceptin/OFQ (NOPr) | 79 ± 18 |
| SNC80 (DOR) | UK14,304 (α2AR) | 84 ± 30 |
| SNC80 (DOR) | Clonidine (α2AR) | 72 ± 21 |
| SNC80 (DOR) | CP 55,9140 (CB1) | 88 ± 32 |
| SNC80 (DOR) | 8-OH-DPAT (5-HT1A) | 119 ± 12 |
| Nociceptin/OFQ (NOPr) | UK14,304 (α2AR) | 108 ± 15 |
| Nociceptin/OFQ (NOPr) | Clonidine (α2AR) | 119 ± 11 |
| Nociceptin/OFQ (NOPr) | CP 55,9140 (CB1) | 119 ± 7 |
| Nociceptin/OFQ (NOPr) | 8-OH-DPAT (5-HT1A) | 124 ± 10 |