Substrates of UGT3A2 expressed in HEK293T cells
UGT3A2 activities were assayed as described under Materials and Methods.
| Activity | ||
|---|---|---|
| UDP Glucose | UDP Xylose | |
| pmol · min−1 · mg lysate protein−1 | ||
| Xenobiotic | ||
| Drug | ||
| Mycophenolic acid | 453 | 607 |
| Paracetamol | − | N.D. |
| Phenobarbital | − | N.D. |
| Sulfamethoxazole | − | N.D. |
| Ibuprofen | − | N.D. |
| Ketoprofen | − | N.D. |
| Tetracycline | − | N.D. |
| Chloramphenicol | − | N.D. |
| Bioflavones | ||
| Naringenin | 842 | 470 |
| Genistein | 659 | 523 |
| Chrysin | 403 | 352 |
| Isorhamnetin | 358 | N.D. |
| Biochanin A | 278 | 246 |
| Acacetin | 257 | N.D. |
| Diadzein | 235 | N.D. |
| Formononetin | 100 | N.D. |
| 4′-Hydroxyflavanone | 39 | N.D. |
| 2′-Hydroxyflavanone | 33 | N.D. |
| Primuletin | 28 | N.D. |
| Baicalein | − | N.D. |
| Epigallocatechin gallate | − | N.D. |
| Morin | − | N.D. |
| 4′-Hydroxyflavone | + | N.D. |
| 2′-Hydroxyflavone | + | N.D. |
| 3-Hydroxyflavone | − | N.D. |
| Kaempferol | + | N.D. |
| Quercetin | − | N.D. |
| Luteolin | + | N.D. |
| Epichatechin | − | N.D. |
| Other | ||
| 4-Methylumbelliferone | 959 | 686 |
| 1-Hydroxypyrene | 865 | 462 |
| 7-Hydroxycoumarin | 760 | 311 |
| 8′-Hydroxyquinoline | 594 | 222 |
| 1-Naphthol | 570 | 205 |
| 4-Nitrophenol | 225 | 82 |
| Phenolthalein | 21 | N.D. |
| (−)Borneol | − | N.D. |
| Steroids | ||
| 17α-Ethinylestradiol | 104 | N.D. |
| 17β-Estradiol | 86 | N.D. |
| Diethylstilbestrol | 66 | N.D. |
| Estrone | 44 | N.D. |
| Estriol | 39 | N.D. |
| 17α-Estradiol | 32 | N.D. |
| Androsterone | − | N.D. |
| Testosterone | − | N.D. |
| Dihydrotestosterone | − | N.D. |
| Etiocholanolone | − | N.D. |
| Bile Acids | ||
| Ursodeoxycholic acid | − | N.D. |
| Chenodeoxycholic acid | − | N.D. |
| Lithocholic acid | − | N.D. |
| Hyodeoxycholic acid | − | N.D. |
| α-Muricholic acid | − | N.D. |
| β-Muricholic acid | − | N.D. |
+, low activity detected but not quantifiable by the thin-layer chromatography method; −, no activity; N.D., not determined.