TABLE 3

Kinetic constants of the simulated curves for CPR-CYP1A2 vesicle systems

Apparent Km (Kmapp) and Vmax values for CYP1A2 metabolism of 7-ER and 7-EFC were determined from the data in Fig. 6, using DynaFit (BioKin). These data represent the mean ± S.E.M. for four determinations.

Vesicles7-ER7-EFC
KmappVmaxKmappVmax
μMpmol/min/pmol 1A2μMpmol/min/ pmol A2
PC0.07 ± 0.02102 ± 150.043 ± 0.008130 ± 11
ER lipids0.0078 ± 0.003*109 ± 70.013 ± 0.008*161 ± 10
DRM0.0033 ± 0.001*86 ± 70.0015 ± 0.001***171 ± 11

  • ↵* P ≤ 0.05, significantly different from PC.

  • ↵*** P ≤ 0.001, significantly different from PC.