KD-SR | koff-SR | kon-SR | |
---|---|---|---|
nM | s−1 | M−1 · s−1 | |
Wild type | 140 | 15.9 ± 0.8 | 1.2 ± 0.1 × 108 |
D163A | 110 | 10.3 ± 0.7* | 9.7 ± 0.6 × 107 |
R120A | 100 | 11.5 ± 0.4* | 1.2 ± 0.1 × 108 |
R120A/D163A | 100 | 11.1 ± 0.9* | 1.2 ± 0.1 × 108 |
↵* P < 0.05, significant differences between control and mutant parameters were calculated using ANOVA with a Dunnett's post test.