TABLE 1

Intrinsic properties of the hKv1.3 wild-type channel and the single (Hp56T), double (Hp56T/Ai11C), and triple (Hp56T/Ci10A/Ai11C) mutant channels transiently transfected in COS7 cells

All values are given as mean ± S.D.; numbers of independent experiments are given in parentheses. Values for the wild-type, the single-mutant, and the double-mutant channels are from Dreker and Grissmer (2005).

PropertiesWild TypeHp56THp56T/Ai11CHp56T/Ci10A/Ai11C
V1/2, mV−33 ± 1 (2)−36 ± 1 (9)−37 ± 1 (3)−10 ± 3 (4)
K, mV10 ± 1 (2)7 ± 1 (9)7 ± 1 (3)11 ± 1 (4)
τm, ms2.9 ± 0.4 (6)3.9 ± 0.7 (10)4.9 ± 1.1 (10)4.5 ± 1.2 (4)
τh at +40 mV, ms291 ± 53 (6)678 ± 134 (10)1220 ± 200 (10)1943 ± 1300 (4)
τt at −60 mV, ms117 (1)434 ± 119 (8)30 ± 14 (9)32 ± 2 (2)
τrec at −120 mV, s13.6 ± 0.2 (4)≤ 1.1 (4)≤ 1.1 (4)N.D.
  • N.D., not determined.