TABLE 1

Dose-response data for GlyRs with conventional mutations at positions 63, 159, and 207

Data are presented as mean ± S.E.M. (n = 3–8).

	Glycine	β-Alanine	Taurine
WT EC₅₀	100 ± 13	303 ± 61	522 ± 75
R_max		0.9 ± 0.03	0.9 ± 0.04
F63A EC₅₀	89,000 ± 6000	115,000 ± 8,000	>500,000
R_max		0.4 ± 0.05
F63Y EC₅₀	2,000 ± 400	23,000 ± 3,000	N.R.
R_max		1.0 ± 0.08
F159A EC₅₀	8,100 ± 200	104,000 ± 17,000	126,000 ± 32,000
R_max		1.1 ± 0.05	0.9 ± 0.07
F159Y EC₅₀	13.2 ± 1.0	9.3 ± 0.7	23.3 ± 4
R_max		1.4 ± 0.04	1.0 ± 0.11
F207A EC₅₀	N.R.	N.R.	N.R.
R_max
F207Y EC₅₀	161 ± 23	520 ± 88	754 ± 122
R_max		0.6 ± 0.06	0.7 ± 0.04

R_max, ratio of maximal currents induced by β-alanine or taurine versus glycine; N.R., no response at 100 mM.