[3H]QNB | pKB | |||||
---|---|---|---|---|---|---|
Bmax | pKA | ACh | Pilocarpine | NDMC | McN-A-343 | |
fmol/mg protein | ||||||
M4 WT | 159 ± 28 | 10.45 ± 0.12 | 4.89 ± 0.02 | 5.01 ± 0.13 | 6.66 ± 0.09 | 4.92 ± 0.02 |
M4 D1063.26A | 41 ± 20* | 9.70 ± 0.06* | 3.95 ± 0.09* | 4.24 ± 0.16* | 6.67 ± 0.13 | 4.70 ± 0.14 |
M4 W1083.28A | 111 ± 21 | 9.77 ± 0.13* | 4.01 ± 0.06* | 4.59 ± 0.07 | 7.08 ± 0.03* | 4.88 ± 0.04 |
M4 L1093.29A | 107 ± 31 | 9.12 ± 0.10* | 3.11 ± 0.09* | 4.22 ± 0.11* | 5.93 ± 0.02* | 4.83 ± 0.17 |
M4 D1123.32N | 183 ± 12 | 8.44 ± 0.09* | <2 | <3 | <4 | <3 |
M4 D1123.32E | 152 ± 39 | 10.34 ± 0.10 | 3.19 ± 0.02* | 4.16 ± 0.09* | 6.52 ± 0.04 | 4.62 ± 0.02 |
M4 S1163.36A | 158 ± 18 | 10.51 ± 0.07 | 3.61 ± 0.10* | 5.09 ± 0.23 | 6.63 ± 0.02 | 5.07 ± 0.03 |
M4 N1173.37A | 260 ± 48 | 9.95 ± 0.10 | 3.64 ± 0.04* | 4.46 ± 0.04 | 6.18 ± 0.11* | 5.09 ± 0.03 |
M4 V1203.40A | 264 ± 34* | 9.41 ± 0.16* | 5.63 ± 0.05* | 5.95 ± 0.03* | 6.07 ± 0.04* | 5.30 ± 0.03 |
M4 D1293.49E | 77 ± 40 | 10.14 ± 0.13 | 5.04 ± 0.07 | 4.53 ± 0.19 | 6.69 ± 0.09 | 4.80 ± 0.15 |
M4 D1293.49N | 54 ± 9* | 10.11 ± 0.17 | 5.54 ± 0.10* | 5.08 ± 0.09 | 6.57 ± 0.05 | 5.05 ± 0.08 |
↵* p < 0.01, significantly different from WT value as determined by one-way ANOVA with Dunnett's post hoc test.