TABLE 1

Oligonucleotide primers

Primer pair 1, amplification of the BmP2X wild-type sequence to introduce a mammalian Kozak sequence. Primer pairs 2–9, BmP2X-HdP2X chimera generation. Region refers to the amino acid positions encoded in the resulting PCR product. EarI endonuclease recognition sites incorporated into primers are in bold.

Forward Primer (5′-3′)Reverse Primer (5′-3′)Region
1BmP2XGCCGCCACCATGGGCTTGAACTGCGGCATACACTTGAGTGCTCG1–414
2BmP2X N-TM2GCCGCCACCATGGGCTTGAACAACTCTTCACAGCAGGCAGTTAAGAACAACAAG1–378
3HdP2X N-TM2GCCGCCACCATGACGAATTTCACTAATACGAACTCTTCACTTCAGGAAGTCCGTTAAAAAGTC1–371
4BmP2X TM1-CAACTCTTCACGTATCGGCGTGCTCAACAGGCTCAGTGCTCGTTAGCATGCTCC28–414
5HdP2X TM1-CAACTCTTCAAAGTTGGGCGCCATCAATCGGACGGAGTTTTGGTAGCGCACAG40–480
6BmP2X NGCCGCCACCATGGGCTTGAACAACTCTTCACTTTTTGTTGCCGATGTGGAC1–28
7HdP2X NGCCGCCACCATGACGAATTTCACTAATACGAACTCTTCAACGAGTGCTGTAGACCTTGAC1–40
8BmP2X CAACTCTTCAAAGCGTCGTGATCTGTACAAGAGTGCTCGTTAGCATGCTCC378–414
9HdP2X CAACTCTTCAAAGCGTCGTGATCTGTACAAGGGAGTTTTGGTAGCGCACAG371–480

  • N, amino termini; C, carboxyl termini.