TABLE 2

Properties of ATP-evoked currents in wild-type, mutant, and chimeric P2X channels

Values are means ± S.E.M. n ≥ 15 for each channel.

Channel	Peak Current	10–90% Rise Time	T.10%	I₂₀
	nA	ms		%
WT BmP2X	−14,677 ± 882^††	951 ± 129^††	17,016 ± 2420^††	86.6 ± 1.7^††
WT HdP2X	−1808 ± 141^**	65 ± 2^**	46 ± 3^**	0.1 ± 0.08^**
Chimera A	−77 ± 6^**^††	269 ± 14^**^††	364 ± 37^**^††	34.8 ± 1.7^**^††
Chimera B	−1282 ± 165^**	281 ± 8^**^††	237 ± 10^**^††	2.9 ± 0.2^**^††
Chimera C	−63 ± 4^**^††	1730 ± 192^††	10,020 ± 1391^††	82.4 ± 1.5^††
Chimera D	−22 ± 1^**^††	195 ± 12^**	61 ± 7^**	52.7 ± 3.5^**^††
Chimera E	−5441 ± 538	1072 ± 70^††	1388 ± 92^**^††	10.6 ± 1.1^**^††
Chimera F	−196 ± 12^**^††	482 ± 39^††	1882 ± 249^**^††	54.7 ± 1.7^*^††
Ser414Δ	−14,056 ± 628^††	1515 ± 230^††	16,244 ± 1374^††	88.6 ± 1.6^††
Arg413Δ	−11,720 ± 883^††	588 ± 75^††	6469 ± 766^**^††	68.3 ± 3.3^**^††
Leu412Δ	−8600 ± 1043^**^††	454 ± 39^††	700 ± 41^**^††	5.1 ± 1.1^**^††
Phe410Δ	−8670 ± 857^**^††	491 ± 31^††	764 ± 41^**^††	8.4 ± 1.1^**^††
Glu408Δ	−15,812 ± 1152^††	358 ± 23^**^††	1118 ± 113^**^††	13.2 ± 1.7^**^††
Lys400Δ	−7078 ± 864^**^††	276 ± 35^**^††	620 ± 77^**^††	9.8 ± 1.7^**^††
Glu394Δ	−14,263 ± 1194^††	328 ± 14^**^††	1337 ± 140^**^††	26.1 ± 2.6^**^††
Tyr388Δ	NF	NF	NF	NF
Y411A	−14,379 ± 580^††	731 ± 113^††	16,207 ± 4615^††	79.0 ± 3.8^††
R413K	−11,640 ± 1168^††	1500 ± 313^††	15,455 ± 3934^††	84.5 ± 3.0^††
R413A	−7805 ± 1476^**^††	624 ± 91^††	2964 ± 396^**^††	43.4 ± 5.7^**^††
R413D	−7435 ± 871^*^††	275 ± 24^**^††	758 ± 50^**^††	40.0 ± 3.2^**^††

NF, nonfunctional.
↵* P < 0.05; ** P < 0.01, significant difference from wild-type BmP2X.
↵†† P < 0.01, significant difference from wild-type HdP2X.