TABLE 1

Effect of curcumin on cell division and morphology of mitotic spindles

T24 cells were treated with curcumin for 48 h or transfected with Aurora-A siRNA. The percentage of mitotic cells and mitotic phenotype was scored based on Aurora-A or γ-tubulin fluorescence in addition to DNA morphology by immunofluorescent microscopic analysis (the number of cells for each condition was larger than 300), using Aurora-A siRNA as a positive control. Data were a quantification of Fig. 3 and Supplemental Fig. 2. Results are representative of three independent experiments.