Voltage-dependence of activation and steady-state fast inactivation for Nav1.7 wild-type and PEPD mutant channels
The voltage dependence of activation was examined using a series of 50 ms depolarizing test pulses from −80 mV to +60 mV. The midpoint of activation was estimated by fitting the data with a Boltzmann function. The voltage dependence of steady-state fast inactivation was examined using a series of 200-ms conditioning prepulses from −120 to +30 mV, followed by a 20-ms test pulse to +15 mV to assess channel availability. The midpoint of inactivation was estimated by fitting the data with a Boltzmann function.
| V1/2 | n | ||
|---|---|---|---|
| Activation | Inactivation | ||
| mV | |||
| Wild-type | |||
| DMSO [0.1% (v/v)] | −10.6 ± 0.8 | −58.8 + 1.5 | 10 |
| T1464I | |||
| DMSO [0.1% (v/v)] | −11.7 ± 0.6 | −49.0 ± 0.5* | 9 |
| M1627K | |||
| DMSO [0.1% (v/v)] | −10.2 ± 0.6 | −37.1 ± 0.5*† | 10 |
* P < 0.05 from wild-type by unpaired Student's t test.
† P < 0.05 from T1464I by unpaired Student's t test.