Holo-P450 yields for enzymes expressed in E. coli
Expression levels shown as mean ± S.D. (n ≥ 3).
| Enzyme | Yield |
|---|---|
| pmol P450/mg | |
| CYP1A1 | 141 ± 8 |
| S116A | 103 ± 7 |
| S122A | 56 ± 2 |
| S122T | 61 ± 0.5 |
| F123A | 9 ± 3 |
| E161K | 94 ± 9 |
| E166Q | 33 ± 7 |
| V191M | 225 ± 9 |
| F224A | 54 ± 12 |
| V228T | 95 ± 11 |
| E256K | 115 ± 36 |
| Y259F | 115 ± 5 |
| N309T | 132 ± 2 |
| L312F | 115 ± 4 |
| D313A | 57 ± 3 |
| D313N | 35 ± 3 |
| G316V | 165 ± 13 |
| A317G | 101 ± 5 |
| D320A | 223 ± 9 |
| T321G | 59 ± 2 |
| T321P | 59 ± 12 |
| T321S | 57 ± 2 |
| V322A | 27 ± 9 |
| I386G | 22 ± 7 |
| I386V | 42 ± 32 |
| I458P | 134 ± 26 |
| I458V | 89 ± 6 |
| T497S | 84 ± 5 |