TABLE 1

Effect of bharangin on apoptosis induced by TNF-α and chemotherapeutic agents in tumor cells

Cells were pretreated with the indicated concentrations of bharangin, and then incubated with chemotherapeutic agents (1 nM TNF-α, 0.1 μM 5-FU, and 10 μg/ml thalidomide) for 24 h. Cell viability was analyzed by measuring mitochondrial dehydrogenase activity. Values given are mean ± S.D. of four replicates.

Cell Lines	Cell Viability
	Medium	TNF-α				5-FU				Thalidomide
	2 μM BG	0 μM BG	0. μM BG5	1 μM BG	2 μM BG	0 μM BG	0.5 μM BG	1 μM BG	2 μM BG	0 μM BG	0.5 μM BG	1 μM BG	2 μM BG
	%
KBM-5	96 ± 2.5	95 ± 5.4	88 ± 6.6	64 ± 3.3^*	49 ± 3.4^*	96 ± 3.9	91 ± 3.7	71 ± 5.2^*	53 ± 5.5^*	94 ± 7.0	87 ± 3.4	70 ± 3.6^*	49 ± 4.7^*
Jurkat	94 ± 4.3	95 ± 3.5	90 ± 3.1	63 ± 4.1^*	40 ± 5.7^*	97 ± 3.1	90 ± 3.4	74 ± 4.1^*	49 ± 2.2^*	96 ± 2.9	88 ± 3.3	76 ± 4.3^*	47 ± 3.5^*
U-937	93 ± 8.5	94 ± 2.9	84 ± 2.7^*	66 ± 2.2^*	43 ± 2.8^*	95 ± 2.5	89 ± 4.6	72 ± 4.1^*	54 ± 2.8^*	92 ± 4.9	83 ± 3.5^*	64 ± 5.9^*	42 ± 2.9^*
K-562	92 ± 5.5	95 ± 2.4	91 ± 3.2	56 ± 2.5^*	43 ± 3.5^*	94 ± 6.2	87 ± 3.2^*	59 ± 5.4^*	49 ± 3.4^*	96 ± 3.0^*	80 ± 3.9	63 ± 2.9^*	50 ± 4.1^*
U-266	95 ± 4.0	97 ± 3.2	86 ± 4.4^*	63 ± 2.9^*	47 ± 3.6^*	95 ± 1.9	88 ± 3.0	74 ± 4.7^*	59 ± 2.7^*	94 ± 5.1	89 ± 5.1	79 ± 5.8^*	56 ± 3.9^*
MM0.1S	94 ± 6.7	94 ± 4.7	89 ± 6.8	74 ± 5.6^*	53 ± 3.0^*	94 ± 3.1	86 ± 5.9^*	79 ± 3.3^*	54 ± 3.2^*	94 ± 4.8	87 ± 5.4^*	74 ± 3.0^*	51 ± 3.3^*
RPMI-8226	92 ± 4.8	94 ± 4.9	85 ± 7.0^*	67 ± 2.0^*	44 ± 5.8^*	95 ± 2.1	83 ± 3.6^*	69 ± 3.0^*	51 ± 5.5^*	96 ± 3.3	87 ± 5.1^*	74 ± 3.0^*	52 ± 3.3^*
HL-60	91 ± 7.3	96 ± 2.3	87 ± 2.3^*	73 ± 2.1^*	50 ± 2.8^*	95 ± 2.3	88 ± 2.8	74 ± 2.5^*	47 ± 2.6^*	94 ± 4.6^*	84 ± 2.9	72 ± 3.3^*	46 ± 2.9^*

↵* P < 0.05, significance of difference compared with control.
BG, bharangin; 5-FU, 5-fluorouracil.