Rectification in ionic gradients
The intracellular and extracellular concentrations of chloride were varied to shift the reversal potential and to induce Goldman rectification. Decreasing the intracellular chloride concentration (I2/E1) was sufficient to enhance outward rectification significantly and to decrease the intercept of the rectification profile. Outward rectification at low Po persisted in all ion gradient conditions, as shown by the slopes of the rectification profiles.
| Solutions | ECl | Erev | Slope | Intercept | n |
|---|---|---|---|---|---|
| mV | mV | ||||
| I1/E1 | −7.75 | −9.32 ± 0.55 | 0.37 ± 0.03 | 0.67 ± 0.04 | 34 |
| I1/E2 | +57.0 | 40.5 ± 4.53 | 0.43 ± 0.02 | 0.64 ± 0.04 | 6 |
| I2/E1 | −21.6 | −22.3 ± 2.31 | 0.39 ± 0.04 | 0.56 ± 0.05* | 8 |
↵* P < 0.05, compared with I1/E1, Student's t test.