TABLE 1

Primer and probe sequences used in quantitative real-time RT-PCR assays to measure mRNA levels of lipogenic genes

Transcript quantitation based on real-time monitoring of amplification was carried out according to the parameters defined under Materials and Methods.

Gene Name or Symbol	GenBank Accession No.	Primers (5′→3′)		TaqMan Probe (5′→3′)
Gene Name or Symbol	GenBank Accession No.	Forward	Reverse	TaqMan Probe (5′→3′)
SCD1	NM_005063	`CACCTCTTCGGATATCGTCCT`	`GTAGTTGTGGAAGCCCTCACC`	FAM-`ATGACAAGAACATTAGCCCCCGGGAG`-BHQ
ACSL1	NM_001995	`AGATCTTGCAGTAATTTGTTTCACAA`	`CGCTCACTATGTTTCGGTGAGT`	FAM-`TGGAACTACAGGCAACCCCAAAGGAG`-BHQ
DGAT1	NM_012079	`TGGAACATCCCTGTGCACA`	`TGCCCCGTCGAAGCAT`	FAM-`TGGTGCATCAGACACTTCTACAAGCCC`-BHQ
Adfp	NM_001122	`GTGACTGGCAGTGTGGAGAAG`	`TCCGACTCCCCAAGACTGT`	FAM-`CCAAGTCTGTGGTCAGTGGCAGCA`-BHQ
PPARγ	NM_138712	`ATGCTGGCCTCCTTGATGA`	`GCTTTCGCAGGCTCTTTAGAA`	FAM-`TCTCATATCCGAGGGCCAAGGCTTC`-BHQ
Cyclophilin	NM_021130	`ACGGCGAGCCCTTGG`	`TTTCTGCTGTCTTTGGGACCT`	FAM-`CGCGTCTCCTTTGAGCTGTTTGCA`-BHQ

FAM, 5-carboxyfluorescein; BHQ, black hole quencher.