TABLE 1

Compositions of intracellular and extracellular electrophysiological saline solutions containing standard and low chloride concentrations

The concentration of each reagent is listed for the four electrophysiological saline solutions used in this study, together with the final concentration of acid or base used to adjust intracellular solutions to a pH of 7.2 and extracellular solutions to a pH of 7.4. Also shown are the final concentrations of monovalent anions and cations for each solution. Intracellular and extracellular solutions were adjusted to a final osmolarity of 315 and 325 mOsM, respectively, with sucrose or deionized water as necessary.

Concentration
I1I2E1E2
mM
Solute
    NaCl1605
    KCl1206833
    Sodium gluconate155
    Potassium gluconate48
    MgCl22211
    CaCl21.51.5
    HEPES10101010
    EGTA1010
    d-Glucose66
pH correction
    HCl0.20.2
    NaOH12.512.5
Final ion composition
    Cl1247216813
    Na+ + K+133129163163
  • I, intracellular; E, extracellular; 1, standard chloride concentration; 2, low chloride concentration.