Primer sequence used for quantitative RT-PCR analysis
| Direction | Sequences (5′-3′) |
|---|---|
| Human CYP1A2 | |
| Forward | 5′-CATCCC CCACAGCACAACAA-3′ |
| Reverse | 5′-TCCCACTTGGCCAGGACTTC-3′ |
| Human CYP2C9 | |
| Forward | 5′-AGCTTGGAAAACACTGCAGT-3′ |
| Reverse | 5′-CCTGCTGAGAAAGGCATGAAG-3′ |
| Human CYP2C19 | |
| Forward | 5′-CCTGCTGAGAAAGGCATGAAG-3′ |
| Reverse | 5′-CCTGCTGAGAAAGGCATGAAG-3′ |
| Human CYP2D6 | |
| Forward | 5′-CCCATATGACATCCCGTGACATC-3′ |
| Reverse | 5′-TTGGTGATGAGTGTCGTTCCCTTA-3′ |
| Human β-ACTIN | |
| Forward | 5′-GACAGGATGCAGAAGGAGATTACT-3′ |
| Reverse | 5′-TGATCCACATCTGCTGGAAGGT-3′ |
| Mouse Cyp2d9 | |
| Forward | 5′-TGGCACAGATAGAGAAGGCCA-3′ |
| Reverse | 5′-TCACGCACCACCATGAGC-3′ |
| Mouse Cyp2d22 | |
| Forward | 5′-GACACCCTTTCAGCCCTAACA-3′ |
| Reverse | 5′-GAAGCGTGGGTCATCGTACT-3′ |
| Mouse β-Actin | |
| Forward | 5′-CCAGGGTGTGATGGTGGGAA-3′ |
| Reverse | 5′-TTCACGGTTGGCCTTAGGGT-3′ |