IC50 values of PCNA-I1 and PCNA-I2 (μM)
Cells were plated into 96-well plates at 1000 to 5000 cells/well. After an overnight incubation, the cells were treated for 4 days with various concentrations (up to 10 μM) of PCNA-I1 or PCNA-I1. The live cells were stained with MTT and counted as described under Materials and Methods. IC50 values are derived from growth inhibition curves. Data shown for tumor cells are mean of five to seven experiments and for primary cells are mean of three experiments. The means of IC50 for tumor cells and normal cells are 0.17 ± 0.07 and 1.60 ± 0.36, respectively (P < 0.0001).
| Species, Tissue Origin, and Cell line | PCNA-I1 | PCNA-I2 |
|---|---|---|
| μM | ||
| Tumor cell lines | ||
| Human | ||
| Breast | ||
| MCF-7 | 0.15 | 1.01 |
| T47D | 0.15 | N.D. |
| Prostate | ||
| PC-3 | 0.24 | 0.97 |
| DU145 | 0.16 | 1.19 |
| 22Rv1 | 0.18 | N.D. |
| LAPC-4 | 0.30 | 1.79 |
| LNCaP | 0.14 | 0.56 |
| Melanoma | ||
| A375 | 0.16 | 3.75 |
| MDA-MB435 | 0.29 | N.D. |
| Mouse | ||
| Prostate | ||
| TRAMP-C2RE3 | 0.20 | N.D. |
| Melanoma | ||
| B16 | 0.14 | N.D. |
| K1735 | 0.05 | N.D. |
| Fibrosarcoma | ||
| UV2237 | 0.25 | N.D. |
| Colon cancer | ||
| CT26-P | 0.13 | N.D. |
| CT26-R100 | 0.08 | N.D. |
| CT26-R500 | 0.07 | N.D. |
| Mean ± SD | 0.17 ± 0.07 | N.D. |
| Normal cells | ||
| Human | ||
| Blood vessel | ||
| HUVEC | 1.54 | N.D. |
| Bone marrow | ||
| Mesenchymal | 0.99 | N.D. |
| Stem cells | ||
| Breast | ||
| Epithelial cells | 1.67 | N.D. |
| Prostate | ||
| Epithelial cells | 2.00 | N.D. |
| Mouse | ||
| Bone marrow | ||
| Stroma cells | 1.90 | N.D. |
| Spleen | ||
| Lymphocytes | 1.50 | N.D. |
| Mean ± SD | 1.60 ± 0.36 | N.D. |
N.D., not determined.