Potency of probe ligands in affecting GTPγS binding
Values represent EC50 ± S.E.M. for three to four independent experiments conducted in triplicate.
| Mutant | EC50 | |||
|---|---|---|---|---|
| WT | S5.42A | S5.43A | S5.46A | |
| nM | ||||
| DNS | 290 ± 70 | — | 135 ± 25 | — |
| DNX | 550 ± 30 | — | 69 ± 17* | 660 ± 250 |
| DHX | 370 ± 180 | 1300 ± 410 | 92 ± 28 | 6600 ± 6200 |
| Quinpirole | 740 ± 120 | 1700 ± 860 | 203 ± 5 | 7200 ± 1900* |
| Dopamine | 1700 ± 1040 | 10,700 ± 1500* | 4300 ± 1600 | — |
↵* Significantly different from WT (P < 0.05; one-way ANOVA and Tukey-Kramer multiple comparisons test; —, potency cannot be measured because no functional activity was detected.