Primer sequences for real-time quantitative reverse transcription-PCR (human)
| Gene | Sequence (5′→3′) | Amplicon Size |
|---|---|---|
| bp | ||
| LXRα1 | ||
| Forward | CTG CGA TCG AGG TGA TGC TT | 197 |
| Backward | ACT ACG GCT CAA ACG GAA C | |
| LXRα3 | ||
| Forward | TGA AGC GGC AAG AGG AGG AA | 166 |
| Backward | AGC TCA GTG CCA CTA CGA AG | |
| LXRα4 | ||
| Forward | CGT TTG AGG TTT GCT GCT TG | 233 |
| Backward | ACT ACG GCT CAA ACG GAA C | |
| LXRα5 | ||
| Forward | CTG CGA TCG AGG TGA TGC TT | 155 |
| Backward | GTT AGT ACC GAG TTA CGT CG | |
| GAPDH | ||
| Forward | ACT TCG CTC AGA CAC CAT GG | 139 |
| Backward | GGG AAG TAA CTG GAG TTG ATG |
GAPDH, glyceraldehyde-3-phosphate dehydrogenase.