Structural determinants of subunit selectivity
Fitted IC50 values were determined from composite concentration-effect curves constructed from recordings in 4 to 41 oocytes (n) as described under Materials and Methods at a holding potential of −40 mV; the steady-state response was fixed at 0 for all conditions except GluN2B wild type. Data for wild-type GluN2B from Table 2 are included here for comparison.
| IC50 | IC50 Mutant/IC50 Wild Type | n | |
|---|---|---|---|
| μM | |||
| GluN1/GluN2A(2B-loop) | 48 | 1.03 | 20 |
| GluN1/GluN2A(2B-loop,S232T) | 53 | 1.14 | 8 |
| GluN1/GluN2A(2B-loop,I176Y) | 36 | 0.77 | 9 |
| GluN1/GluN2A(2B-loop,L238Y) | 50 | 1.06 | 8 |
| GluN1/GluN2A | 47 | 9 | |
| GluN1/GluN2A(2B-ATD-LBD linker) | 49 | 1.1 | 11 |
| GluN1/GluN2B | 0.10 | 41 | |
| GluN1/GluN2B(2A-ATD-LBD linker) | 0.14 | 1.4 | 4 |
| GluN1/GluN2D | 205 | 9 | |
| GluN1/GluN2D(2B-ATD-LBD linker) | 155 | 0.75 | 14 |