Agonist | Wild Type | lev-8(x15) | acr-8(ok1240) | lev-8(ok1519); acr-8(ok1240) | ||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
O1 (area) | O2 (area) | n | O1 (area) | O2 (area) | n | O1 (area) | O2 (area) | n | O1 (area) | O2 (area) | n | |
ms | ms | ms | ms | |||||||||
ACh | ||||||||||||
0.1 μM | 0.23 ± 0.06 | 0.49 ± 0.1 | 6 | 0.37 ± 0.08 | 1.1 ± 0.05 | 5 | N.M. | N.M. | N.M. | N.M. | ||
(0.81) | (0.19) | (0.81) | (0.19) | |||||||||
1 μM | 0.27 ± 0.06 | 0.53 ± 0.1 | 7 | 0.42 ± 0.12 | 1.2 ± 0.5 | 7 | 0.26 ± 0.06 | 0.44 ± 0.1 | 7 | 0.28 ± 0.08 | N.D. | 7 |
(0.83) | (0.17) | (0.74) | (0.25) | (0.86) | (0.13) | (1) | ||||||
50 μM | 0.24 ± 0.03 | 0.43 0.06 | 4 | 0.32 ± 0.05 | 0.7 ± 0.12 | 7 | N.M. | N.M. | 0.32 ± 0.09 | N.D. | 4 | |
(0.88) | (0.12) | (0.84) | (0.16) | (1) | ||||||||
100 μM | 0.21 ± 0.04 | 8 | 0.33 ± 0.05 | 5 | 0.24 ± 0.04 | N.D. | 3 | 0.19 ± 0.05 | N.D. | 8 | ||
(1) | N.D. | (1) | N.D. | (1) | (1) | |||||||
300 μM | 0.14 ± 0.02 | 4 | 0.18 ± 0.02 | 4 | N.M. | N.M. | N.M. | N.M. | ||||
(1) | N.D. | (1) | N.D. | |||||||||
Levamisole | ||||||||||||
0.1 μM | 0.18 ± 0.02 | 0.44 ± 0.12 | 8 | 0.33 ± 0.06 | 0.80 ± 0.11 | 6 | N.M. | N.M. | N.M. | N.M. | ||
(0.85) | (0.15) | (0.86) | (0.14) | |||||||||
1 μM | 0.24 ± 0.05 | N.D. | 6 | 0.40 ± 0.13 | 0.98 ± 0.09 | 4 | 0.29 ± 0.06 | N.D. | 7 | 0.26 ± 0.06 | N.D. | 6 |
(1) | (0.79) | (0.21) | (1) | (1) | ||||||||
100 μM | 0.27 ± 0.04 | N.D. | 4 | 0.21 ± 0.03 | 5 | 0.27 ± 0.04 | N.D. | 3 | 0.16 ± 0.07 | N.D. | 11 | |
(1) | (1) | N.D. | (1) | (1) |
n, number of recordings for each condition; N.M., not measured; N.D., not detected.