TABLE 2

Summary of the observed temperature effects on α7 PAM activity from whole-cell electrophysiology experiments

DrugAverage absolute value (pA)Normalized to average peak at 23.5°C
23.5°C37°C
1 mM ACh (n = 7)643 ± 132294 ± 550.474 ± 0.032
Type I PAM
    100 μM ACh and 1 mM 5-HI (n = 12)1346 ± 158455 ± 700.330 ± 0.024
    100 μM ACh and 10 μM NS-1738 (n = 6)3602 ± 2941244 ± 2760.339 ± 0.052
Type II PAM
    100 μM ACh and 10 μM PNU-120596 in ES (n = 8)1333 ± 414104 ± 310.117 ± 0.028
    100 μM ACh and 1 μM PNU-120596 in ES (n = 9)509 ± 13644 ± 70.129 ± 0.029
    100 μM ACh and 10 μM PNU-120596 in ESBSA (n = 11)2753 ± 284**392 ± 97*0.148 ± 0.030
    100 μM ACh and 10 μM TQS (n = 7)3080 ± 4841438 ± 2290.464 ± 0.025
  • ES, external solution; ESBSA, external solution containing 30 μM BSA.

  • * P < 0.05. The effect of 30 μM BSA in external solution to increase the 100 μM ACh and 10 μM PNU-120596 response at 37°C is statistically significant by comparing the average responses from 11 cells recorded in ESBSA with the average responses of 8 cells recorded in ES.

  • ** P < 0.01. The effect of 30 μM BSA in external solution to increase the 100 μM ACh and 10 μM PNU-120596 response at 23.5°C is statistically significant by comparing the average responses from 11 cells recorded in ESBSA with the average responses of 8 cells recorded in ES.