TABLE 2

Kinetic analysis of block and recovery from block by PeIA[S9H] and PeIA[V10A]

Data are means ± S.E.M. from three to nine oocytes. Numbers in parentheses are 95% confidence intervals.

	k_off	k_obs	k_on	K_i
	min⁻¹	min⁻¹	min⁻¹ M⁻¹	M⁻⁹
α3β2
PeIA[S9H]	0.062 ± 0.001 (0.059–0.064)	0.18 ± 0.01 (0.161–0.204)	0.118 ± 0.01 × 10⁹^{^a}	0.525^{^b}
PeIA[V10A]	0.456 ± 0.008 (0.440–0.472)	0.55 ± 0.04 (0.46–0.62)	0.94 ± 0.04 × 10⁸^{^a}	4.85^{^b}
α6/α3β2β3
PeIA[S9H]	0.11 ± 0.01 (0.10–0.12)	0.31 ± 0.01 (0.29–0.34)	0.200 ± 0.01 × 10⁹^{^a}	0.550^{^b}
α3β4
PeIA[S9H]	0.032 ± 0.001 (0.030–0.034)	0.088 ± 0.002 (0.084–0.093)	0.56 ± 0.002 × 10⁶^{^a}	57^{^b}
α6/α3β4
PeIA[S9H]	0.078 ± 0.001 (0.075–0.081)	0.21 ± 0.01 (0.184–0.240)	0.132 ± 0.01 × 10⁸^{^a}	5.9^{^a}