Solutions used for cell isolation and electrophysiological recordings
| Low Ca+2 Tyrode | Whole-Cell Recording | ||
|---|---|---|---|
| Internal | 5 K+ External | 140 K+ External | |
| mM | |||
| 137 NaCl | 100 K+ aspartate | 135 NaCl | 140 KCl |
| 2.7 KCl | 30 KCl | 5.4 KCl | 10 HEPES |
| 0.008 CaCl2 | 5 HEPES | 0.33 NaH2PO4 | 1 MgCl2 |
| 0.88 MgCl2 | 1 MgCl2 | 5 HEPES | 0.1 CaCl2 |
| 0.36 NaH2PO4 | 10 EGTA | 1 MgCl2 | 1 TEA |
| 12 NaHCO3 | 0.1 ATP Na2 | 2 CaCl2 | |
| 5.5 glucose | 5.5 glucose | ||
TEA, tetraethylammonium chloride.