Various agonists displacing fMLFIIK-FITC binding to mFpr1-RBL or mFpr2-RBL cells with different affinity
Shown are the means of IC50 values and Hill coefficient (nH) of unlabeled ligands in competition binding assays conducted with at least 3 independent experiments as described.
| Agonist | mFpr1-RBL | mFpr2-RBL | ||
|---|---|---|---|---|
| IC50 (M) | nH | IC50 (M) | nH | |
| WKYMVm | 1.2 ± (0.06) × 10−8 | 1.2 ± 0.1 | 7.1 ± (1.6) × 10−7 | 0.6 ± 0.1 |
| fMLF | 4.7 ± (0.98) × 10−5 | 1.0 ± 0.1 | ND | ND |
| fMLFK | 4.1 ± (17) × 10−4 | 1.1 ± 0.5 | > 5.6 × 10−5 | ND |
| fMLFE | 8.8 ± (1.1) × 10−6 | 0.6 ± 0.1 | ND | ND |
| fMLFW | 1.5 ± (0.05) × 10−7 | 0.9 ± 0.03 | ND | ND |
| fMLFII | 5.4 ± (0.43) × 10−9 | 0.8 ± 0.03 | 3.8 ± (0.72) × 10−6 | ND |
| fMIFL | 4.2 ± (0.57) × 10−7 | 0.7 ± 0.1 | ND | ND |
| fMIVTLF | 2.4 ± (0.24) × 10−8 | 0.8 ± 0.06 | 6.0 ± (4.0) × 10−6 | 0.5 ± 0.2 |
| fMMYALF | 2.7 ± (0.22) × 10−7 | 0.7 ± 0.04 | ND | ND |
| fMLFIIK | 2.2 ± (0.14) × 10−7 | 0.8 ± 0.03 | 6.4 ± (2.7) × 10−6 | 0.9 ± 0.1 |
| MLFIIK | ND | ND | ND | ND |
| Quin C1 | 8.5 ± (0.52) × 10−6 | 0.6 ± 0.06 | > 1.4 × 10−4 | ND |
| Compound 43 | 7.8 ± (0.28) × 10−6 | 0.7 ± 0.04 | > 6.1 × 10−4 | ND |
ND, not determined.