TABLE 1

MDA-7/IL-24 synergizes with HDACIs to kill primary human GBM cells

GBM6 cells were plated as single cells (500–2500 per 60-mm dish, in sextuplicate). Twelve hours after plating, the cells were treated with GST or GST-MDA-7 (10–30 nM), SBHA (1–3 μM), or sodium valproate (Na Val, 0.5–1.5 mM), as indicated. Forty-eight hours later, the cells were washed free of drugs, and the colonies were permitted to form for 20 days (n = 3 ± S.E.M.). Using the Calcusyn for Windows program, we calculated the fraction affected (Fa) and the combination index (CI). A CI value of less than 1.00 indicates a synergy of interaction.