TABLE 3

Molecular species of phospholipid fatty acids during lipoapoptosis and protection by UDCA-LPE

Same treatment as in Table 2. Data in nmol/mg protein, mean ± S.D., N = 4.

PL Class	# Carbon and Double Bonds^{^a}	Con	Pal	Pal	UDCA-LPE	Changes	PL Species^{^b}
PL Class	# Carbon and Double Bonds^{^a}	Con	Pal	+ UDCA-LPE	UDCA-LPE	Pal + UDCA-LPE vs. Pal	PL Species^{^b}
PC	PC 30:0	0.13 ± 0.01	0.24 ± 0.01^*	0.20 ± 0.00^*^†	0.09 ± 0.01^*	↓	14:0,16:0
	PC 32:2	0.08 ± 0.00	0.12 ± 0.0001^*	0.14 ± 0.01^*^†	0.05 ± 0.01^*	↓	16:1,16:1; 14:0,18:2
	PC 32:0	1.17 ± 0.05	5.92 ± 0.22^*	4.63 ± 0.11^*^†	0.67 ± 0.08^*	↓	16:0,16:0
	PC O 34:2	0.09 ± 0.00	0.11 ± 0.0001^*	0.13 ± 0.00^*^†	0.07 ± 0.01^*	↑	16:1,18:1; 16;0,18:2
	PC O 34:1	0.20 ± 0.01	0.24 ± 0.01^*	0.26 ± 0.01^*	0.13 ± 0.02^*	ns	16:0,18:1, 18:0;16:1
	PC O 34:0	0.05 ± 0.00	0.10 ± 0.0001^*	0.09 ± 0.00^*^†	0.03 ± 0.00^*	↓	16:0,18:0
	PC 34:3	0.53 ± 0.01	0.68 ± 0.02^*	0.79 ± 0.01^*^†	0.38 ± 0.05^*	↑	16:0,18:3; 16:1,18:2
	PC 34:2	12.53 ± 0.27	14.28 ± 0.57^*	15.64 ± 0.67^*	8.99 ± 1.35^*	ns	16:1,18:1; 16;0,18:2
	PC 34:1	9.06 ± 0.25	11.23 ± 0.32^*	14.00 ± 0.48^*^†	7.79 ± 1.05^*	↑	16:0,18:1, 18:0;16:1
	PC 34:0	0.30 ± 0.02	0.39 ± 0.02^*	0.27 ± 0.01^†	0.15 ± 0.03^*	↓	16:0,18:0
	PC O 36:5	0.03 ± 0.00	0.03 ± 0.0001	0.04 ± 0.01^*^†	0.02 ± 0.004	↑	16:1,20:4; 16:0,20:5
	PC O 36:4	0.05 ± 0.00	0.05 ± 0.0001	0.06 ± 0.00^*^†	0.04 ± 0.007^*	↑	18:1,18:3; 16:0,20:4
	PC O 36:2	0.43 ± 0.02	0.46 ± 0.01^*	0.5 ± 0.02^*	0.27 ± 0.04^*	ns	18:0,18:2; 18:1,18:1
	PC 36:5	0.29 ± 0.02	0.34 ± 0.01^*	0.42 ± 0.01	0.25 ± 0.04	↑	16:1,20:4; 16:0,20:5
	PC 36:2	5.07 ± 0.09	5.31 ± 0.18	6.05 ± 0.26^*^†	4.17 ± 0.58^*	↑	18:1,18:1; 18:0,18:2
	PC 36:1	1.27 ± 0.05	1.27 ± 0.03	1.48 ± 0.07^*^†	1.22 ± 0.17	↑	18:0,18:1; 16:0,20:1
	PC 38:6	4.53 ± 0.20	5.63 ± 0.28^*	6.20 ± 0.27^*	4.96 ± 0.72	ns	16:1,22:5; 16:0,22:6
	PC 38:5	1.04 ± 0.05	1.19 ± 0.05^*	1.46 ± 0.04^*^†	1.13 ± 0.17	↑	18:1,20:4; 16:0,22:5
	PC 40:5	0.29 ± 0.01	0.32 ± 0.01^*	0.39 ± 0.02^*^†	0.31 ± 0.04	↑	18:0,22:5
PE	PE 32:2	0.01 ± 0.00	0.03 ± 0.0001^*	0.03 ± 0.00^*^†	0.004 ± 0.0001^*	↓	16:1,16:1; 14:0,18:2
	PE 32:1	0.04 ± 0.00	0.57 ± 0.02^*	0.37 ± 0.01^*^†	0.02 ± 0.005^*	↓	14:0,18:1, 16:0,16:1
	PE 32:0	0.05 ± 0.00	1.10 ± 0.02^*	0.72 ± 0.02^*^†	0.03 ± 0.003	↓	16:0,16:0
	PE 34:2	0.84 ± 0 .02	1.37 ± 0.05^*	1.54 ± 0.04^*^†	0.61 ± 0.10^*	↑	16:1,18:1; 16;0,18:2
	PE 34:1	0.23 ± 0.01	0.78 ± 0.01^*	1.00 ± 0.03^*^†	0.20 ± 0.02	↑	16:0,18:1, 18:0;16:1
	PE 34:0	0.10 ± 0.01	0.70 ± 0.04^*	0.39 ± 0.02^*^†	0.07 ± 0.01	↓	16:0,18:0
	PE 36:3	0.42 ± 0.02	0.47 ± 0.02	0.58 ± 0.03^*^†	0.42 ± 0.07	↑	18:1,18:2; 18:0,18:3
	PE 36:1	0.16 ± 0.00	0.15 ± 0.01	0.19 ± 0.01^†	0.18 ± 0.04	↑	18:0,18:1; 16:0,20:1
	PE 38:6	3.31 ± 0.13	4.69 ± 0.22^*	5.22 ± 0.24^*	3.46 ± 0.50	ns	16:1,22:5; 16:0,22:6;18:1,20:4
PS	PS 38:6	0.20 ± 0.01	0.33 ± 0.02^*	0.42 ± 0.03^*^†	0.35 ± 0.03^*	↑	16:1,22:5;16:0,22:6;18:1,20:4
	PS 38:5	0.07 ± 0.01	0.09 ± 0.01^*	0.12 ± 0.01^*^†	0.07 ± 0.01	↑	18:1,20:4; 16:0,22:5
	PS 38:4	1.38 ± 0.03	1.27 ± 0.05	1.43 ± 0.06	1.60 ± 0.19^*	ns	18:0,20:4
	PS 40:6	2.58 ± 0.14	3.20 ± 0.16^*	4.32 ± 0.14^*^†	4.11 ± 0.55^*	↑	18:1,22:5: 18:0,22:6
	PS 40:5	0.21 ± 0.04	0.22 ± 0.02	0.33 ± 0.03^*^†	0.19 ± 0.03	↑	18:0,22:5
	PS 42:6	0.00 ± 0.00	0.02 ± 0.0001	0.08 ± 0.01^*^†	0.06 ± 0.04^*	↑	18:1,24:5; 18:0,24:6
PI	PI 34:1	0.05 ± 0.00	0.17 ± 0.01^*	0.22 ± 0.02^*^†	0.05 ± 0.01	↑	16:0,18:1, 18:0;16:1
	PI 36:1	0.08 ± 0.02	0.13 ± 0.01	0.23 ± 0.02^*	0.08 ± 0.01^*	ns	18:0,18:1; 16:0,20:1
	PI 38:5	0.98 ± 0.03	1.04 ± 0.1	1.20 ± 0.08^*	1.33 ± 0.18^*	ns	18:1,20:4; 16:0,22:5
LPE	LPE 16:0	0.46 ± 0.07	0.72 ± 0.02^*	0.79 ± 0.03^*^†	0.31 ± 0.05^*	↑
	LPE 18:2	0.04 ± 0.007	0.048 ± 0.005	0.06 ± 0.007^*^†	0.04 ± 0.007	↑
	LPE 18:1	0.18 ± 0.034	0.18 ± 0.01	0.24 ± 0.009^*^†	0.17 ± 0.04	↑
	LPE 18:0	0.97 ± 0.17	1.33 ± 0.06^*	1.6 ± 0.07^*^†	0.85 ± 0.12	↑
	LPE 20:4	0.07 ± 0.009	0.08 ± 0.006	0.09 ± 0.007^*^†	0.08 ± 0.01	↑
	LPE 20:0	0.05 ± 0.01	0.05 ± 0.001	0.06 ± 0.004^*^†	0.04 ± 0.004	↑

Con, control; ns, not significant.
↵^a Species annotation is based on the assumption that only even acyl chains are present.
↵^b The fatty acid combination are based on the fatty acids detected by GC-MS (compare with Table 1).
↵* P < 0.05, con versus Pal, Pal + UDCA-LPE or UDCA-LPE; ^†P < 0.05, Pal versus Pal + UDCA-LPE.