Cytostatic activity of purine nucleoside analogs in MCF-7 and MCF-7.Hyor cells in the absence or presence of the selective purine nucleoside phosphorylase inhibitor DADMe-Imm-H
Results are the mean ± S.D. of at least two independent experiments.
| IC50 | ||||
|---|---|---|---|---|
| MCF-7/0 | MCF-7.Hyor | |||
| As Such | + DADMe-Imm-H | As Such | + DADMe-Imm-H | |
| µM | ||||
| 2-Chloro-2′-deoxyadenosine (cladribine) | 1.3 ± 0.4 | 1.1 ± 0.3 | 13 ± 1 | 0.69 ± 0.22 |
| 2-Fluoroadenine-arabinofuranoside (fludarabine) | 39 ± 12 | 31 ± 11 | 0.30 ± 0.07 | 22 ± 7 |
| 2-Fluoro-2′-deoxyadenosine | 2.9 ± 0.6 | 3.3 ± 0.3 | 0.19 ± 0.01 | 2.3 ± 0.1 |
| Methyl(talo)-F-Ado | 46 ± 24 | 78 ± 9 | 3.4 ± 0.4 | 95 ± 17 |
| 6-Methylpurine-2′-deoxyriboside | 55 ± 5 | 57 ± 10 | 1.2 ± 0.5 | 61 ± 26 |
| 6-Mercaptopurine-riboside | 0.75 ± 0.22 | 25 ± 1 | 2.2 ± 1.0 | 4.1 ± 3.0 |
| 6-Thioguanosine | 0.44 ± 0.21 | >100 | 1.3 ± 0.4 | 62 ± 42 |
| 8-Aminoguanosine | 79 ± 0.1 | >100 | >100 | >100 |
| 5,6-Dichlorobenzimidazole ribofuranoside | 14 ± 2 | 12 ± 3 | 13 ± 3 | 12 ± 0.1 |
| 2-Amino-2′-deoxy-2′-fluoro-adenosine | >100 | >100 | >100 | >100 |
| Adenine-arabinofuranoside | >100 | — | >100 | — |
—, not determined.