Rate of Response Increase Mean ± S.E.M. | Rate of Response Decay Mean ± S.E.M. | |
---|---|---|
arbitrary area U/min | ||
Control*,** | ||
Deltorphin II (n = 3) | 0.47 ± 0.01a | −0.087 ± 0.02b (0.0023) |
SB235863 (n = 7) | 0.46 ± 0.02 | −0.0008 ± 0.02 (0.9712) |
mcp-TIPP (n = 6) | 0.40 ± 0.02c | −-0.022 ± 0.01 (0.1491) |
SNC-80 (n = 8) | 0.34 ± 0.03 | −0.048 ± 0.01d (0.0015) |
Morphine (n = 8) | 0.33 ± 0.02 | −0.034 ± 0.01e (0.0399) |
DPDPE (n = 7) | 0.31 ± 0.02 | −0.13 ± 0.02 (P < 0.0001) |
Met-enkephalin (n = 8) | 0.27 ± 0.01 | −0.11 ± 0.01 (P < 0.0001) |
Dynasore***,**** | ||
Deltorphin II (n = 3) | 0.51 ± 0.03 (P < 0.001) | 0.00 ± 0.01 (P = 0.83)f,g |
SB235863 (n = 6) | 0.50 ± 0.04 (P < 0.001)h | 0.01 ± 0.01 (P = 0.66)i |
mcp-TIPP (n = 6) | 0.59 ± 0.04 (P < 0.001)j | 0.00 ± 0.01 (P = 0.92)k |
SNC-80 (n = 4) | 0.56 ± 0.03 (P < 0.001) | 0.01 ± 0.02 (P = 0.47)l |
Morphine (n = 3) | 0.30 ± 0.02 (P < 0.001) | −0.01 ± 0.01 (P = 0.29) |
DPDPE (n = 3) | 0.42 ± 0.04 (P < 0.001) | −0.08 ± 0.02 (P = 0.0017) |
Met-enkephalin (n = 4) | 0.33 ± 0.03 (P < 0.001) | −0.05 ± 0.01 (P = 0.0005)g |
↵a Deltorphin II versus SNC-80, P < 0.001.
↵b Deltorphin II versus SB 235863, P < 0.05.
↵c mcp-TIPP versus met-enkephalin, P < 0.0001. Decrease rate (P < 0.0001).
↵d SNC-80 versus met-enkephalin, P < 0.001.
↵e Morphine versus met-enkephalin, P < 0.01.
↵f SB235863 versus met-enkephalin, P < 0.05.
↵g Decay rates obtained in control and dynasore-treated cells were compared by two-way ANOVA, which showed an effect of treatment (P < 0.01). Post hoc comparisons indicated that decay rate for met-enkephalin and deltorphin II were significantly reduced by dynasore treatment (P < 0.05).
↵h SB235863 versus met-enkephalin, P < 0.01.
↵i mcp-TIPP versus DPDPE, P < 0.01.
↵j mcp-TIPP versus DPDPE, P < 0.01. Decrease rate (P < 0.0001).
↵k DPDPE versus Deltorphin II, P < 0.05.
↵l Met-enkephalin versus SNC-80, P < 0.05.
↵* All slopes for response increase were different from zero as calculated by linear regression (P < 0.0001); **P values corresponding to slopes for response decrease are indicated in brackets. Differences in increase and decrease rates were established by one way ANOVA indicating: increase rate (P < 0.0001); ***Slopes for response increase rate were different from zero as calculated by linear regression (P < 0.001); ****P values for slopes of response decay are indicated in parentheses. Differences in increase and decrease rates were established by one-way ANOVA indicating the following: increase rate (P < 0.0001).