Primers used
| Gene | Forward | Reverse | Assay |
|---|---|---|---|
| mATF3 | TTGGATCCATGATGCTTCAACAC | CCCAAGCTTTTACTTGTCATCGTC | Q-PCR |
| GAPDH | GGAGCCAAACGGGTCATCATCTC | GAGGGGCCATCCACAGTCTTCT | Q-PCR |
| mα-MHC | AGAAGCCCAGCGCTCCCTCA | TGCCTCGGGTCAGCTGGGAA | Q-PCR |
| mβ-MHC | AACCTGTCCAAGTTCCGCAAGGTG | GAGCTGGGTAGCACAAGAGCTACT | Q-PCR |
| mANF | CTCCGATAGATCTGCCCTCTTGAA | GGTACCGGAAGCTGTTGCAGCCTA | Q-PCR |
| mBNP | GCTCTTGAAGGACCAAGGCCTCAC | GATCCGATCCGGTCTATCTTGTGC | Q-PCR |
| mBECN (−182) | CAGGGGTCCTTGCGTCAGCTCCCGTC | GACGGGAGCTGACGCAAGGACCCCTG | EMSA |
| mBECN (−164) | CTCCCGTCGTGACGTCACTTCTGGTC | GACCAGAAGTGACGTCACGACGGGAG | EMSA |
| Beclin-1 promoter | CCGCTCGGCCTTTGGATGGC | ACCCAGGCTCGTTCTACCGC | ChIP |
ANF, atrial natriuretic factor; BNP, brain natriuretic peptide; ChIP, chromatin immunoprecipitation; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; m, mouse; mBECN, mouse Beclin-1; Q-PCR, quantitative PCR.