Primers | |
---|---|
PCR for cloning | |
2B7-1R | ATACGGTACCTGGTGCAATGCAATGCTTG |
2B7-240F | GCTAGGTACCGCTGAAGGATAGCACTCATA |
2B7-283F | GCATGGGTACCTTGCATAAGACAGATGGCAT |
2B7-1046F | ATAGACGCGTGACCTGAAGGAATACCTCAA |
2B7-2076F | ATGTACGCGTGCCGGTTTCTAAGGCATATA |
2B7-3026F | ATAGACGCGTTCAGCCTCCCACTTTGCAAT |
2B7-4026F | ATCAACGCGTAGTGATGCTTCAGGAAACTT |
2B7-4926F | TACTACGCGTGAGCCCTGTAAACTCTTAAT |
Mutagenesis | |
2B7-MT1F | GACACTCTTCTAAAATGCAGTGCATAAGACAGATG |
2B7-MT1R | CATCTGTCTTATGCACTGCATTTTAGAAGAGTGTC |
2B7-MT2F | CTAAAATATATTGCATGGATCAGATGGCATGTCCA |
2B7-MT2R | TGGACATGCCATCTGATCCATGCAATATATTTTAG |
2B7-MT3F | TTGCATAAGACAGATACAGTGTCCATACAAGATC |
2B7-MT3R | GATCTTGTATGGACACTGTATCTGTCTTATGCAA |
2B7-MT4F | ATGTCCATACAAGATGGCAGATATTAGCTGAAGG |
2B7-MT4R | CCTTCAGCTAATATCTGCCATCTTGTATGGACAT |
2B7-MT5F | TACAAGATCCTTGATCGGTGCTGAAGGATAGCAC |
2B7-MT5R | GTGCTATCCTTCAGCACCGATCAAGGATCTTGTA |
qPCR | |
CYP3A4F | AGTATGGAAAAGTGTGGGGCT |
CYP3A4R | TGGAGACAGCAATGATCGTAA |
p21F | AGACTCTCAGGGTCGAAAAC |
p21R | TAAGGCAGAAGATGTAGAGC |
ChIP-qPCR | |
2B7-7906/-7747F | CCTTCCTTTGGATTTTGGCC |
2B7-7906/-7747R | TGAATGACCTGGGGTGAATT |
2B7-355/-209F | GTTTTGTGTCAAATGGACTGC |
2B7-355/-209R | CCCTTTTATGTTTATGAGTGC |
2B7-3026/-2907F | ATAGACGCGTTCAGCCTCCCACTTTGCAAT |
2B7-3026/-2907R | GCCACTCAGGCACGTGTAGA |
CYP3A4F | TGGCAGAACTTGCCTTCAATT |
CYP3A4R | TGCAGTTGGTGTTGTTCTGCA |
+1, nucleotides of the primers are numbered from the UGT2B7 translation start site ATG codon with the A; F, forward; R, reverse; MT, mutation.