Surface expression of δ subunits for receptors containing mutated subunits
The surface enzyme-linked immunosorbent assay signal relative to wild-type [mean ± S.E. (no. of transfections)] is shown for the subunit combinations shown in the first column. In each group, significance levels were computed using a one-way analysis of variance with Bonferroni correction over positive control (wild-type), and the three or four subunit combinations shown. Results are shown for the difference to wild-type expression. The receptors containing both mutated α4 and δ subunits were compared as described in Results.
| Subunits Expressed | Surface Expression |
|---|---|
| α4(A)-β2-δ | 1.75 ± 0.21 (16)** |
| α4(B)-β2-δ | 1.69 ± 0.25 (14)* |
| α4(AB)-β2-δ | 2.51 ± 0.35 (4)** |
| α4(C)-β2-δ | 3.25 ± 0.44 (8)*** |
| α4-β2-δ(A) | 1.03 ± 0.48 (5) (ns) |
| α4-β2-δ(B) | 0.55 ± 0.08 (6)* |
| α4-β2-δ(C) | 0.26 ± 0.06 (5)*** |
| α4(B)-β2-δ(B) | 0.53 ± 0.27 (3) |
| α4(C)-β2-δ(C) | 1.49 ± 0.16 (6) |
ns, P > 0.05; *P < 0.05; **P < 0.01; ***P < 0.001.