Agonist Concentration | Amplitude | Slope | ||
---|---|---|---|---|
DAMGO | Morphine | DAMGO | Morphine | |
nM | RVU | RVU/min | ||
1 | 50 ± 4 | 30 ± 2 | 41 ± 2 | 32 ± 2 |
10 | 99 ± 6* | 87 ± 7*** | 65 ± 4* | 62 ± 5*** |
100 | 137 ± 11*** | 131 ± 8*** | 58 ± 4 | 64 ± 4*** |
1000 | 118 ± 12*** | 116 ± 10*** | 54 ± 8 | 58 ± 6** |
The signal amplitude and initial slope were calculated for the SPR signals obtained after stimulation with increasing concentrations of morphine and DAMGO. RVU is an arbitrary unit (1 RVU = 0.001 unit of reflectance). The results are reported as the mean ± S.E.M (n = 5 to 6).
↵* P < 0.05; **P < 0.01; ***P < 0.001 (one-way ANOVA followed by a Bonferroni’s post hoc test, compared with 1 nM results).