Pharmacological Inhibitor | Amplitude | Slope | ||
---|---|---|---|---|
DAMGO | Morphine | DAMGO | Morphine | |
RVU | RVU/min | |||
Control | 137 ± 11 | 131 ± 8 | 58 ± 4 | 64 ± 4 |
Dynasore | 112 ± 3* | 100 ± 4** | 66 ± 4 | 60 ± 5 |
CTX | 92 ± 4*** | 70 ± 4*** | 52 ± 2 | 44 ± 2** |
U0126 | 108 ± 4>* | 80 ± 5*** | 63 ± 3 | 50 ± 4 |
The signal amplitude and slope were calculated for the SPR signals obtained after a stimulation with 100 nM agonist with indicated pretreatments. RVU is an arbitrary unit (1 RVU = 0.001 unit of reflectance). The results are reported as the mean ± S.E.M (n = 5 to 6).
↵* P < 0.05; **P < 0.01; ***P < 0.001 (one-way ANOVA followed by a Dunnett’s post hoc test, compared with control results).