Construct | [3H]INCB3344 Displacement by INCB3344 | [3H]CCR2-RA Displacement by CCR2-RA-[R] | ||
---|---|---|---|---|
WT CCR2 | 7.8 ± 0.03 | (16) | 7.8 ± 0.05 | (16) |
K72A2.34 | 7.8 ± 0.01 | (15) | 7.7 ± 0.1 | (21) |
D78N2.40 | 7.8 ± 0.1 | (16) | 7.7 ± 0.1 | (21) |
I208A5.45 | 7.8 ± 0.1 | (17) | 7.7 ± 0.1 | (19) |
V244A6.36 | 8.1 ± 0.03* | (7.5) | no binding | |
E291A7.39 | 6.9 ± 0.1* | (126) | 7.7 ± 0.1 | (21) |
Y305A7.53 | 7.7 ± 0.03 | (19) | no binding | |
K311A8.49 | 7.7 ± 0.03 | (18) | 6.8 ± 0.1* | (169) |
F312A8.50 | 8.0 ± 0.1* | (9.2) | no binding |
↵* P < 0.005 versus WT CCR2, Student’s t test.