TABLE 2

Threshold for ethanol sensitivity in α1 and α2 GlyR USERs is bimodal and lower than WT

α1 And α2 GlyR USERs exhibited a bimodal pattern in response to ethanol. Two threshold concentrations are denoted for USERs to represent the bimodal effect at ultra-low and higher (>3 mM) ethanol concentrations. The threshold for ethanol sensitivity was reduced from 30 mM ethanol in α1 GlyR WT to 0.075 mM in α1 GlyR USERs 1 and 2, and 0.05 mM in α1 GlyR USER 3. At higher ethanol concentrations, α1 GlyR USERs 1 and 2 exhibited a threshold of 3 mM ethanol, whereas α1 GlyR USER 3 exhibited a threshold of 10 mM ethanol. In α2 GlyR USERs, thresholds were significantly reduced from 50 mM ethanol in α2 GlyR WT to 0.25 and 10 mM in α2 GlyR USER 1. Each data point represents the mean ± S.E.M. from at least four to sixteen oocytes. All threshold values were statistically significant with P < 0.05.