Effects of PYD-106 on single channel properties
All values are mean ± S.E.M., All patches contained 2 or more channels (n = 4 patches). Intervals either adjacent to or containing a double opening were omitted from analysis. nPo is the product of the number of channels in the patch and the open probability for an individual channel, which is the probability of at least one channel being open.
| Control | + 100 μM PYD-106 | |
|---|---|---|
| nPo | 0.032 ± 0.015 | 0.067 ± 0.024* |
| nPo (% control) | 100 | 257 ± 45%* |
| Opening frequency (Hz) | 0.086 ± 0.045 | 0.159 ± 0.069 |
| Opening frequency (% control) | 100 | 217 ± 34%* |
| Mean shut time | 24.3 ± 10.6 | 10.0 ± 3.2 |
| Mean open time | 0.35 ± 0.07 | 0.48 ± 0.07* |
| Open τ 1, ms (%) | 0.19 ± 0.07 (39) | 0.25 ± 0.11 (42) |
| Open τ 2, ms (%) | 0.46 ± 0.10 (61) | 0.65 ± 0.16 (58) |
| Amplitude 1 (pA) | 2.58 ± 0.11 | 2.40 ± 0.25 |
| Amplitude 2 (pA) | 3.31 ± 0.16 | 3.30 ± 0.16 |
| Conductance 1 (pS) | 32.3 ± 1.4 | 29.9 ± 3.1 |
| Conductance 2 (pS) | 41.4 ± 2.0 | 41.3 ± 2.0 |
↵* P < 0.05, paired t test.