Summary of LGR4 ECD binding data for wild-type, chimeric, and mutant R-spondins
The number of observations is indicated in parentheses.
| R-spondin | pKI ± S.E.M. |
|---|---|
| MBP-Th-RSPO1 | 6.90 ± 0.08*** (3) |
| MBP-Th-RSPO2 | 7.26 ± 0.13*** (4) |
| MBP-Th-RSPO3 | 7.31 ± 0.12*** (3) |
| MBP-Th-RSPO4 | 8.12 ± 0.07 (6) |
| MBP-Th-RSPO2-4 | 8.36 ± 0.04 (3) |
| MBP-Th-RSPO4-1 | 7.32 ± 0.11*** (4) |
| MBP-Th-RSPO2 T111I | 7.57 ± 0.12 (3) |
↵*** P < 0.001 versus MBP-Th-RSPO4 by one-way ANOVA with Tukey’s multiple comparison test, except for RSPO2 T111I, which was compared with RSPO2.