Isothermal titration calorimetry and spectral titration derived thermodynamic parameters of interaction
| Enzyme | ΔH | ΔS | ΔG | N | KDa | KS | ΔFh |
|---|---|---|---|---|---|---|---|
| kcal/mol | cal/mol per K | kcal/mol | unit-less | 10−6 M | % | ||
| 2A6b | −5.8 (0.9)d | 5.3 (3.2) | −7.3 (0.1) | 1.0 (0.1) | 4.20 (0.86) | 5.22 (0.40) | 87.0 (14.9) |
| 2B6b | −8.2 (1.0) | 4.6 (1.5) | −9.5 (0.6) | 1.1 (0.1) | 0.08 (0.02) | 0.75 (0.32) | 62.0 (2.0) |
| 2B6c | −6.3 (2.2) | 3.2 (2.3) | −7.5 (1.4) | 1.2 (0.3) | 1.82 (0.61) | 1.60 (0.14)e | 42.5 (3.4) |
↵a KA-1 from isothermal titration calorimetry.
↵b Sabinene.
↵c (+)-3-Carene, previously reported values (Wilderman et al., 2013).
↵d Values represent mean of three independent experiments. The confidence interval for P = 0.05 is displayed in parentheses.
↵e Measured using the Hill equation giving the S50 with n = 2.