Comparison of the cAMP dose response induced by NECA and CGS21680 in A2AR mutants
| NECA | CGS21680 | |||
|---|---|---|---|---|
| pEC50a | Change in EC50 Relative to WTb | pEC50a | Change in EC50 Relative to WT | |
| WT | 9.05 ± 0.05 | 1 | 8.27 ± 0.29 | 1 |
| S67A | 8.50 ± 0.14 | 3.4 | 8.40 ± 0.23 | 0.62 |
| E169A | 7.87 ± 0.18**** | 15.1 | 8.07 ± 0.18 | 1.58 |
| H264A | 8.18 ± 0.17** | 7.4 | 7.52 ± 0.20 | 5.62 |
| L267A | 8.81 ± 0.08 | 1.7 | 9.66 ± 0.19** | 0.04 |
WT, wild type.
↵a pEC50 values are expressed as the mean ± S.E.M. calculated from four independent experiments, each performed in triplicate. Values are stated as significantly different from the wild-type receptor using a one-way analysis of variance with Dunnett’s post test.
↵** P < 0.005; ****P < 0.0001.