TABLE 1

Binding properties of AT1 receptor ligands

HEK293 cells stably expressing the AT1 receptor were assayed as described in Materials and Methods. Binding affinities (Kd) are expressed as the means ± S.D. of values obtained in n independent experiments performed in duplicate.

Kdn
nM
AngII1.1 ± 0.312
[Sar1]AngII1.8 ± 0.13
[Ile4]AngII894 ± 1323
[Ile8]AngII3.2 ± 1.13
[Sar1Ile4]AngII78 ± 113
[Sar1Ile8]AngII1.7 ± 0.83
[Ile4Ile8]AngII1254 ± 1984
[Sar1Ile4Ile8]AngII223 ± 383
AngIII5.2 ± 2.14
[Ile4]AngIII3689 ± 20273
[Ile8]AngIII12.3 ± 5.13
[Ile4Ile8]AngIII5783 ± 14083