TABLE 5

Bias factors of AT1 receptor ligands modified at position 1

ΔΔLog(τ/KA) and BF values were calculated as described in Materials and Methods. Data are the mean ± S.E.M. of three to six independent experiments performed in triplicate.

AngII[Sar1]AngIIAngIII
ΔΔLog(τ/KA)BFΔΔLog(τ/KA)BFΔΔLog(τ/KA)BF
EGFR/Gq0.00 ± 0.121.00−0.51 ± 0.180.311.43 ± 0.2126.99
PKC/Gq0.00 ± 0.121.00−0.54 ± 0.160.291.10 ± 0.1612.63
G12/Gq0.00 ± 0.141.00−0.06 ± 0.250.860.80 ± 0.316.35
βArrestin1/Gq0.00 ± 0.191.00−0.19 ± 0.320.650.80 ± 0.276.34
βArrestin2/Gq0.00 ± 0.141.00−0.48 ± 0.130.330.70 ± 0.225.04
EGFR/βarrestin20.00 ± 0.111.00−0.03 ± 0.180.930.73 ± 0.235.36
EGFR/βarrestin10.00 ± 0.171.00−0.33 ± 0.340.470.63 ± 0.274.24
EGFR/G120.00 ± 0.121.00−0.45 ± 0.280.930.63 ± 0.324.26
PKC/βarrestin20.00 ± 0.111.00−0.06 ± 0.150.870.40 ± 0.182.51
PKC/βarrestin10.00 ± 0.171.00−0.36 ± 0.320.440.30 ± 0.181.98
PKC/G120.00 ± 0.121.00−0.48 ± 0.260.330.30 ± 0.291.99
EGFR/PKC0.00 ± 0.091.00−0.03 ± 0.200.940.33 ± 0.232.14
βArrestin1/βarrestin20.00 ± 0.191.000.30 ± 0.311.980.10 ± 0.281.74
βArrestin1/G120.00 ± 0.191.00−0.12 ± 0.380.750.10 ± 0.361.50
βArrestin2/G120.00 ± 0.141.00−0.42 ± 0.250.380.01 ± 0.331.01
  • BF, bias factor.