TABLE 1 Kinetic and steady-state binding parameters of cocaine, MPH, and desipramine
The kon and koff values are derived from the plot of kapp over inhibitor concentration, where the slope and y-intercept of the regression line yield kon and koff, respectively. The kinetic dissociation constant KDkin is koff/kon. The equilibrium dissociation constant KDequ was estimated by a fit of one site-binding model to the amplitudes of the currents that remained unblocked in steady state as a function of increasing inhibitor concentrations.