TABLE 2

Kinetic parameter of metformin uptake in HEK293 cells expressing mouse and human OCT3

Km and Vmax values were determined from nonlinear regression fit of data from Fig. 1 and Supplemental Fig. 1. Data represent mean ± S.D., n = 3 replicates per data point.

hOCT3mOCT3
Km (mM)1.5 ± 0.21.1 ± 0.1
Vmax (nmol/mg protein per minute)10.7 ± 0.666.9 ± 3.5