Voltage dependence of activation and inactivation of mosquito sodium channels
The voltage dependences of conductance and inactivation were fitted with a two-state Boltzmann equation to determine V1/2, the voltage for half-maximal conductance or inactivation, and k, the slope factor for conductance or inactivation. The values in the table represent the mean ± S.E.M., and the number of oocytes was 6–16. The asterisks indicate significant differences as determined by one-way analysis of variance (P < 0.05) with Scheffe’s post hoc analysis.
| Na+ Channel Type | Activation | Inactivation | ||
|---|---|---|---|---|
| V1/2 (mV) | k (mV) | V1/2 (mV) | k (mV) | |
| AaNav1-1 | −29.2 ± 0.8 | 5.2 ± 0.3 | −52.9 ± 0.4 | 4.9 ± 0.2 |
| G1K9A | −32.6 ± 1.2 | 5.0 ± 0.2 | −51.9 ± 0.4 | 4.8 ± 0.1 |
| G1K9I | −32.8 ± 1.4 | 6.3 ± 0.6 | −52.8 ± 1.2 | 4.6 ± 0.2 |
| I1k12N | −33.8 ± 0.9 | 5.1 ± 0.4 | −51.8 ± 0.6 | 4.6 ± 0.1 |
| I1k12N+ G1K9A | −30.2 ± 1.0 | 5.5 ± 0.3 | −55.5 ± 0.8 | 4.8 ± 0.3 |
| I1k12N+ G1K9I | −30.4 ± 0.5 | 6.2 ± 0.2 | −55.5 ± 1.1 | 5.1 ± 0.2 |
| S1o2A | −32.9 ± 0.2 | 5.1 ± 0.1 | −54.0 ± 0.4 | 4.5 ± 0.1 |
| N1o5A | −33.5 ± 0.8 | 4.5 ± 0.2 | −52.1 ± 0.6 | 4.5 ± 0.1 |
| 1o6A | −50.5 ± 1.8* | 6.1 ± 0.6 | −64.7 ± 0.7* | 6.4 ± 0.3 |
| R1o7A | −31.0 ± 0.6 | 5.1 ± 0.3 | −53.9 ± 0.7 | 5.1 ± 0.1 |
| I1i22A | −34.3 ± 1.1 | 5.5 ± 0.4 | −52.9 ± 0.5 | 5.0 ± 0.1 |
| I1i25A | −30.0 ± 0.8 | 7.0 ± 0.6 | −67.5 ± 0.8* | 5.0 ± 0.1 |
| S1i29A | −33.0 ± 0.8 | 5.5 ± 0.4 | −54.8 ± 0.5 | 5.1 ± 0.1 |
| V2i12A | −26.7 ± 0.7 | 5.6 ± 0.4 | −53.5 ± 0.8 | 5.5 ± 0.1 |
| V2i12L | −32.7 ± 0.8 | 5.5 ± 0.1 | −50.9 ± 0.5 | 4.8 ± 0.1 |
| N2i15S | −28.8 ± 1.3 | 5.1 ± 0.1 | −52.9 ± 1.4 | 4.9 ± 0.2 |
| N2i20A | −30.8 ± 0.6 | 5.4 ± 0.2 | −58.7 ± 0.5 | 5.3 ± 0.1 |
| F2i22S | −30.7 ± 0.9 | 5.3 ± 0.7 | −51.3 ± 0.9 | 4.7 ± 0.1 |
| L2i25A | −28.5 ± 0.8 | 5.9 ± 0.3 | −51.5 ± 0.8 | 4.8 ± 0.1 |
| L2i26A | −31.7 ± 1.1 | 7.2 ± 0.4 | −63.1 ± 1.2* | 6.0 ± 0.4 |