Histologic scoring of pancreatic tissues
H&E-stained pancreas sections were observed and scored (0–3) to grade the extent of acinar edema, cell vacuolation, inflammation, and acinar cell necrosis. Data are presented as mean ± S.E.M.
| Groups/Score | Edema (0–3) | Cell Vacuolation (0–3) | Inflammation (0–3) | Necrosis (0–3) |
|---|---|---|---|---|
| Control (n = 7) | 1 ± 0 | 0 ± 0 | 0 ± 0 | 0 ± 0 |
| Cerulein 24 hours (n = 6) | 3 ± 0** | 0 ± 0 | 3 ± 0** | 1.6 ± 0.5** |
| Cerulein 24 hours + TPPU (n = 5) | 0.5 ± 0.7†† | 0 ± 0 | 0.1 ± 0.3†† | 0 ± 0†† |
| Arginine 48 hours (n = 6) | 3 ± 0** | 1 ± 0** | 3 ± 0** | 1 ± 0** |
| Arginine 48 hours + TPPU (n = 8) | 2.5 ± 0.7* | 0 ± 0†† | 2.5 ± 0.7** | 0.8 ± 0.3* |
↵* P < 0.05; **P < 0.01 indicate significant difference between control mice without versus mice with administration of cerulein (24 hours after the initial cerulein injection) and arginine (48 hours after the initial arginine injection).
† P < 0.05; ††P < 0.01 indicate significant difference between TPPU-treated (postinduction of AP with cerulein and arginine) versus non-TPPU–treated mice.