TABLE 3

Alanine substitution introduced into the C-terminal region of UGT2B7

The C-terminal position where the residue(s) was substituted with alanine(s) is shown in italics. This study predicted transmembrane or membrane-bound hydrophobic regions of all mutants by TMHMM Server, version 2.0 (http://www.cbs.dtu.dk/services/TMHMM/), and these regions are shown in underline. The secondary structures of mutants were also predicted by Jpred3 Server (http://www.compbio.dundee.ac.uk/www-jpred/) (Cole et al., 2008), and the residues constituting a helix are shown by brackets. The subscript numbers represent the residue number counted from the N terminus of wild-type UGT2B7.